TABLE 3.
Impact of Amino Acid Substitution of “K” Putative Mutation to Either L, Q, R, Or E On Disease Susceptibility Predicted with The Help of Pmut, SNAP2, Polyphen2, and Mutpred2 tools.
| Substitution | Pmut | SNAP2 | PolyPhen-2 | MutPred2 | Total score |
|---|---|---|---|---|---|
| Ube2C | |||||
| K18L | 0.74 | 1 | 0.005 | 0.772 | 2.517 |
| K18Q | 0.64 | 1 | 0.027 | 0.536 | 2.203 |
| K18R | 0.42 | 1 | 0.32 | 0.38 | 2.12 |
| K18E | 0.66 | 1 | 0.262 | 0.662 | 2.584 |
| K33L | 0.71 | 1 | 0.194 | 0.908 | 2.812 |
| K33Q | 0.59 | 1 | 0.003 | 0.804 | 2.397 |
| K33R | 0.25 | 1 | 0 | 0.681 | 1.931 |
| K33E | 0.59 | 1 | 0.049 | 0.868 | 2.507 |
| Ube2E1 | |||||
| K31L | 0.49 | 1 | 0.037 | 0.156 | 1.683 |
| K31Q | 0.11 | 0 | 0.028 | 0.093 | 0.231 |
| K31R | 0.11 | 0 | 0 | 0.061 | 0.171 |
| K31E | 0.2 | 1 | 0 | 0.113 | 1.313 |
| K24L | 0.28 | 1 | 0.009 | 0.098 | 1.387 |
| K24Q | 0.09 | 0 | 0 | 0.066 | 0.156 |
| K24R | 0.09 | 0 | 0 | 0.044 | 0.134 |
| K24E | 0.11 | 0 | 0.002 | 0.079 | 0.191 |
| K35L | 0.58 | 1 | 0.09 | 0.196 | 1.866 |
| K35Q | 0.47 | 1 | 0.001 | 0.075 | 1.546 |
| K35R | 0.2 | 1 | 0 | 0.052 | 1.252 |
| K35E | 0.47 | 1 | 0.015 | 0.111 | 1.596 |
| K43L | 0.31 | 1 | 0.972 | 0.562 | 2.844 |
| K43Q | 0.2 | 0 | 0.924 | 0.368 | 1.492 |
| K43R | 0.12 | 1 | 0.007 | 0.211 | 1.338 |
| K43E | 0.35 | 1 | 0.896 | 0.369 | 2.615 |
| Ube2H | |||||
| K8L | 0.53 | 1 | 0.016 | 0.872 | 2.418 |
| K8Q | 0.51 | 1 | 0.437 | 0.758 | 2.705 |
| K8R | 0.26 | 1 | 0 | 0.661 | 1.921 |
| K8E | 0.39 | 1 | 0.354 | 0.831 | 2.575 |
| K52L | 0.63 | 1 | 0.82 | 0.943 | 3.393 |
| K52Q | 0.53 | 1 | 0.762 | 0.894 | 3.186 |
| K52R | 0.26 | 1 | 0.001 | 0.821 | 2.082 |
| K52E | 0.57 | 1 | 0.532 | 0.924 | 3.026 |
| Ube2J2 | |||||
| K7L | 0.34 | 1 | 0.032 | 0.481 | 1.821 |
| K7Q | 0.37 | 0 | 0.897 | 0.266 | 1.533 |
| K7R | 0.19 | 0 | 0.868 | 0.205 | 0.395 |
| K7E | 0.33 | 1 | 0.020 | 0.346 | 1.676 |
| K64L | 0.62 | 1 | 1 | 0.704 | 3.324 |
| K64Q | 0.59 | 1 | 0.96 | 0.504 | 3.054 |
| K64R | 0.39 | 0 | 0.542 | 0.208 | 1.14 |
| K64E | 0.59 | 1 | 0.996 | 0.509 | 3.095 |
| K88L | 0.55 | 1 | 0.908 | 0.877 | 3.335 |
| K88Q | 0.48 | 1 | 0.071 | 0.704 | 2.255 |
| K88R | 0.46 | 1 | 0.009 | 0.538 | 2.007 |
| K88E | 0.52 | 1 | 0.503 | 0.812 | 2.835 |
| Ube2S | |||||
| K198L | 0.72 | 1 | 0.999 | 0.567 | 3.286 |
| K198Q | 0.45 | 1 | 0.997 | 0.285 | 2.732 |
| K198R | 0.4 | 1 | 0.996 | 0.186 | 2.582 |
| K198E | 0.44 | 1 | 0.779 | 0.383 | 2.602 |
| K205L | 0.36 | 1 | 0.133 | 0.529 | 2.022 |
| K205Q | 0.37 | 0 | 0.531 | 0.255 | 1.156 |
| K205R | 0.15 | 0 | 0.358 | 0.148 | 0.656 |
| K205E | 0.27 | 1 | 0.187 | 0.349 | 1.806 |
| K210L | 0.73 | 1 | 0.997 | 0.833 | 3.56 |
| K210Q | 0.52 | 1 | 0.999 | 0.559 | 3.078 |
| K210R | 0.29 | 1 | 0.996 | 0.39 | 2.676 |
| K210E | 0.45 | 1 | 0.996 | 0.686 | 3.132 |
| K211L | 0.68 | 1 | 0.997 | 0.683 | 3.36 |
| K211Q | 0.64 | 1 | 0.999 | 0.433 | 3.072 |
| K211R | 0.16 | 1 | 0.996 | 0.2 | 2.356 |
| K211E | 0.52 | 1 | 0.996 | 0.475 | 2.991 |
| K215L | 0.69 | 1 | 0.997 | 0.817 | 3.504 |
| K215Q | 0.7 | 0 | 0.999 | 0.576 | 2.275 |
| K215R | 0.48 | 0 | 0.996 | 0.365 | 1.841 |
| K215E | 0.79 | 1 | 0.996 | 0.664 | 3.45 |
| K216L | 0.88 | 1 | 0.997 | 0.859 | 3.736 |
| K216Q | 0.77 | 1 | 0.999 | 0.639 | 3.408 |
| K216R | 0.74 | 0 | 0.996 | 0.455 | 2.191 |
| K216E | 0.8 | 1 | 0.996 | 0.751 | 3.547 |
*For SNAP2 = Probable Benign: Marked as “0”; Probable damage: Marked as “1”.
*For Pmut, MutPred2, and PolyPhen-2: Effect or Probable damage = >0.5 threshold.
*Gradient of the Green color showed Total confidence score (cumulative score of Pmut, SNAP2, MutPred2, and PolyPhen-2): Higher green color signifies a high confidence score.