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Lytic expression of K8.1. BC-1 and BCBL-1 cells were stimulated with TPA for 0, 24, 48, and 72 h, permeabilized with methanol-ethanol, reacted with rabbit anti-K8.1 antibody, and then incubated with 1 μg of fluoresceinated goat F(ab′)2 anti-mouse total immunoglobulin. Immunofluorescence was detected with an Olympus immunofluorescence microscope.
