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. 2023 Aug 2;18(8):e0287233. doi: 10.1371/journal.pone.0287233

Table 3. Overview on externally validated clinical decision rules (CDRs): Inclusion and exclusion criteria, characteristics and outcomes.

CDR Inclusion criteria Exclusion criteria High risk criteria High risk outcome
Rackoff 1996* Cancer or hematologic
malignancy; fever ≥ 38.5°C once or ≥38.0°C 3× during a 24-h period*; ANC < 0.5 G/l; outpatient
Inpatient onset FN High risk = AMC < 0.1 G/l and temperature
≥39°C
Intermediate risk = AMC < 0.1 G/l and temperature < 39°C
Low risk = AMC ≥ 0.1 G/l
Bacteremia (defined as a positive blood culture)
Klaassen 2000* Cancer or hematologic
malignancy, fever > 38.5°C once or >38.0°C during 12 hour period*; ANC ≤ 0.5 or between 0.5 and 1.0 G/l and expected to fall, outpatient
New diagnosis cancer, HSCT within 6 months,
comorbidity on presentation including severe mucositis and pneumonia
AMC < 0.1 G/l Significant bacterial
Infection—defined as blood or urine culture positive for bacteria, interstitial or lobar consolidation on chest x-ray, or unexpected death from infection (patient not palliative)
Baorto 2001* Cancer or hematologic
malignancy, fever ≥
38.0°C; ANC ≤ 0.5 G/l
Age < 1 y, previous
HSCT
AMC < 0.155 G/l Bacteremia (not defined)
Madsen 2002* Cancer or hematologic
malignancy; fever ≥ 38.5°C once or ≥38.0°C 3× during a 24-h period*; ANC ≤ 0.5 G/l; outpatient
Inpatient onset of FN, HSCT, AML patients in intensive timing theraoy Temperature > = 39.5°C and AMC ≤0.01G/l positive blood culture
Rondinelli 2006* Cancer or hematologic
Malignancy; fever ≥ 38.1°C once or > 37.8°C on 3 separate occasions measured within a period of 24 hours*; ANC (segmented granulocytes and rods) < 0.5 G/l or 0.5–1.0 that tended to drop in 72 hours, outpatient, first episode of FN
HSCT (autolog and allogenic), not the first episode of FN; inpatient onset of FN Score 2.5–5 = low risk
Score 5.5–9 = intermediate risk
Score ≥ 9 = high risk
Age ≤5 y = 1, 2. CVAD = 2, Clinical site = 4.5, Fever >38.5°C = 1, Hemoglobin ≤ 70g/l = 1, upper respiratory tract infection = 2.5
Severe infection complication was defined as the presence of sepsis and/or shock and/or bacteremia or fungemia from blood sample, and/or death from an infectious process during a FN episode.
The presence of any infectious agent in a blood sample was considered as bacteremia.
Septicemia was defined as a syndrome of systemic inflammatory response (involving ≥ 2 of the following characteristics: tachycardia, tachypnea, hypothermia,or hyperthermia, with positive blood culture or clinical and laboratorial infection detected and adequate peripheral perfusion). Patients were considered in septic shock when severe sepsis was observed, with clinical signs of hypoperfusion and blood hypotension, who no longer answered to fluids and who needed inotropic doses to maintain hemodynamic balance.
SPOG-AE (Ammann) 2010* Cancer or hematologic
malignancy; fever ≥ 38.5°C once or ≥38.0°C during ≥2 hours*; ANC ≤ 0.5 G/l; outpatient
Myeloablative chemotherapy; AE known at presentation Applied after 24 hours Total score ≥ 9 = high risk of AE.
Score for preceding chemotherapy
more intensive than ALL
maintenance = 4; Hb ≥ 90 g/l = 5; leukocyte count < 0.3 G/L = 3; platelet count < 50 G/L = 3
Adverse outcome, defined as a SMC (death, complication requiring ICU and potentially life-threatening complication as judged by the treating physician) as a result of infection, MDI (positive bacterial or fungal culture from a normally sterile site and detection of a viral antigen by PCR)
and radiologically confirmed pneumonia. Bacteremia not defined
Hakim 2010* Cancer or hematologic
malignancy; fever ≥ 38.3°C or ≥38.0°C for ≥1 hour*; ANC ≤ 0.5 G/l;
outpatient
HSCT; inpatient
onset FN
Total score ≥ 24 = high risk of invasive
bacterial infection.
Score for cancer diagnosis:
AML = 20, ALL/lymphoma = 7, solids = 0 points; Clinical presentation
serious unwell or toxic = 14 points; Fever ≥ 39°C
at presentation = 11 points; ANC < 0.1 G/l = 10 points
Proven invasive bacterial Infection, defined as isolation of a pathogen
from a sterile body site or as proven by histology.
Culturenegative Sepsis, defined as a systemic response to a possible infection because of hemodynamic instability, focal or multiple organ
involvement or altered mental status or lethargy.
Bacteremia defined as a recognized pathogen cultured from one or more blood cultures or common commensals cultured from two or more blood cultures.
Suttitossatam 2020* fever ≥ 38.3°C or ≥ 38.0°C persisting >1 hour*; ANC < 0.5 G/l or ANC < 1.0 G/l with a predicted decrease to < 0.5 G/l Age < 1 year Age ≥ 10 years Severe adverse outcomes as hypotension (is determined by age and systolic blood pressure, in line with
Pediatric Advanced Life Support (PALS) Guidelines) or shock; respiratory failure (the need for noninvasive
respiratory support or mechanical ventilation); death
AUS (Haeusler) 2020* Cancer or hematologic
Malignancy; fever ≥ 38°C; ANC < 1.0 G/l; in- and outpatient
HSCT in last 3 months;
treatment commenced at a non-participating site
already receiving concurrent intravenous or oral antibiotics (excluding prophylaxis)
Score ≥ 1 = High risk
preceding chemotherapy more intensive than ALL maintenance = 1; WCC < 0.3 G/l = 1; platelet <50 g/L = 1
Three outcomes were analyzed:
Likely bacterial infectionI (any infection with a microbiologically documented bacterial cause or that was clinically documented in categories typically attributed to bacterial infection, including pneumonia, skin and soft-tissue infection, osteomyelitis or myositis, enterocolitis, otitis media or externa, sinusitis, epididymoorchitis, centralvenous catheter pocket or tunnel infection, pharyngitis, perianal abscess or cellulitis, peritonitis or lymphadenitis)
Bacteremia (recognised pathogen (including organisms associated with mucosal barrier injury in the setting of mucositis or neutropenia) from ≥ 1 blood culture set or common commensals from ≥ 2 blood culture sets drawn on separate occasions)
ICU-Admission

* for external crossvalidation with restricted dataframe these definitions were modified for validation because of available data (inclusion of all events which reached the lower temperature limit once).

if not otherwise specified, international consensus definition was used for validation (Haeusler GM, 2015, Pediatr Blood Cancer).

Application at FN presentation for external cross-validation.

Abbreviations: AE, adverse event; ALL, acute lymphoblastic leukemia; AML, acute myeloid leukemia; AMC, absolute monocyte count; ANC, absolute neutrophil count; FN, fever in neutropenia; HSCT, hematopoietic stem cell transplantation; ICU, intensive care unit; MDI, microbiological defined infection; PCR, polymerase chain reaction; SMC, serious medical complication, WCC, white cell count.