Table 1. Oligonucleotides.
vasa sgRNA Forward | TGTAATACGACTCACTATAGCAGCAGCGACGGTAGTGAACGTTTTAGAGCTAGAAATAGC |
mlh3 sgRNA Forward | TGTAATACGACTCACTATAGATCCGAGGCTCTATGCACAGGTTTTAGAGCTAGAAATAGC |
Universal reverse scaffold | AAAAGCACCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAAAAC |
mlh3 ssDNA repair template | TCCAAAAATTTTCCCCTCTGCTACAACAGCTTAATTAAGGTTTAAACGCCATGACTGCATAGAGCCTCGGATCGG |
vasa HRM Forward | CCGAAAATGAACCAGTTAA |
vasa HRM Reverse | CGCTGCTATTACCAGATA |
mlh3 HRM Forward | AAAATTGGAAAACCCTTG |
mlh3 HRM Reverse | AACTATTTCAACCCTGTC |