FIG. 3.

Localization of CD8-TM-CD hybrids in HeLa cells. HeLa cells were transfected with 10 μg of the pCD8-HIV (A), pCD8-HIV-Y712A (B), pCD8-SIV (C), pCD8-SIV-Y721A (D), pCD8-HTLV (E), pCD8-HTLV-Y476S (F), and pCD8-HTLV-Y479S (G) vectors; 48 h later, the cells were fixed, permeabilized, and stained with an anti-CD8-FITC antibody. The distribution of CD8 hybrids was examined by immunofluorescence staining and confocal microscopy analysis. A representative medial section is shown. Scale bar, 20 μm. Data are representative of three independent experiments.