FIG. 7.

Interaction of full-length HIV-1, SIV, and HTLV-1 TM-CDs with in vitro-translated β2-adaptin. β2 subunit was translated in vitro in rabbit reticulocyte lysate and incubated with identical quantities of GST (lane 2), GST-HIV TM-CD (lane 3), GST-SIV TM-CD (lane 4), GST-HTLV TM-CD (lane 5), GST-HIVΔ TM-CD (lane 6), and GST-SIVΔ TM-CD (lane 7). Bound labeled material was analyzed by SDS-PAGE and autoradiography. One-fifth of the input of β2 in vitro-translated product used for the binding assay was run on lane 1.