Fig 3. Profibrotic factors in rPn-stimulated MDMs.

Function of periostin-stimulated monocyte-derived macrophages (MDMs) differentiated after isolation from six healthy controls. (A) The relative gene expression levels of TNF-α, TGF-β, MCP-1, ACTA2, EGR1, and IL-6 in MDM1 and TGF-β, TNF-α, MCP-1, ACTA2, EGR1, and FN1 in MDM2 were determined by quantitative real-time polymerase chain reaction. (B) Protein levels of TNF-α, MCP-1, and IL-6 in MDM1 and MDM2 measured by a bead-based immunoassay. Data are shown as the mean ± standard error of the mean (SEM). Statistical analysis was performed using the Wilcoxon rank-sum test *P < 0.05, n = 6.