TABLE 1.
Score | Scoring Criteria | Scoring Status |
---|---|---|
Optimal | The staining is considered perfect or close to perfect in all of the included tissues. | Sufficient: passed |
Good | The staining is considered fully acceptable in all of the included tissues. However, the protocol may be optimized to improve the proportion and/or staining intensity of cells demonstrated and/or optimize the technical quality as signal-to-noise ratio, morphology, etc. | Sufficient: passed |
Borderline | The staining is considered insufficient, because of a generally too weak staining reaction, a false-negative/false-positive result in one of the included tissues and/or inferior technical quality. The protocol should be optimized. | Insufficient: failed |
Poor | The staining is considered insufficient, because of a false-negative/false-positive result in 2* or more of the included tissues and/or inferior technical quality. The protocol should be optimized. |
Insufficient: failed |
For type 2 IHC assays, 1 false-negative/false-positive result can be scored as poor.