FIG. 8.

VP3 lacks specificity for the 5′-terminal sequences of viral mRNA. (A) The locations of sequences in RNA probes with respect to the wild-type gene 8 mRNA are indicated. The nonviral sequence UUAUU was used to link the 5′ and 3′ gene 8-specific sequences of the probes. ORF, open reading frame. (B) Five picomoles of the RNA probes, g8 5′-3′SacII, g8 5′-3′Eco47III, g8 GG44-86/3′, v-3′60, and nv-60, was incubated alone or with 3.3 μg of VP6-open cores in the presence or absence of 1.7 pmol (1 μg) of luciferase RNA. The upper and lower probe-protein complexes were resolved by electrophoresis and detected by autoradiography. ori, origin.