FIG. 9.

VP3 lacks affinity for the dsRNA genome. ³²P-labeled SP72-v3′40 (50 ng) was incubated alone or with open cores in the absence or presence of either virion-derived dsRNA or luciferase RNA. The reaction mixtures were analyzed by electrophoresis and autoradiography. A phosphorimager was used to quantitate the levels of VP3- and VP1-probe complexes, and the values were adjusted relative to 100% for the reaction mixture containing probe and open cores but no competitor RNA (lane 2). ori, origin.