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. 2023 Jun 23;325(2):H323–H337. doi: 10.1152/ajpheart.00154.2023

Figure 1.

Figure 1.

Experimental design and time line. Two cohorts were included in the experimental design. Rats assigned to cohort 1 were virgin, nonpregnant females implanted with telemeters before mating, followed by repeated intraperitoneal injections of saline (vehicle) or 1 mg/kg ODN2395 during the third trimester of their pregnancy on gestational day (GD) 14, GD16, and GD18, with euthanasia on GD20 to assess maternal hemodynamics, circadian rhythms of maternal blood pressure and heart rate, fetal outcomes, and maternal plasma cytokines. Cohort 2 included pregnant females at GD14 and age-matched virgin, nonpregnant females. Rats assigned to cohort 2 were administered SALINE (vehicle) or 1 mg/kg ODN2395 via intraperitoneal injection and euthanized 4 h after injections to assess maternal plasma cytokines and placental gene expression. TLR, Toll-like receptor 9. *Telemeters were only implanted in cohort 1. Image was created using a licensed version of BioRender. ODN, oligodeoxynucleotides.