Figure 8.

Relationship between placental proinflammatory and clock gene expression in rats administered ODN2395 or saline vehicle on gestational day 14. Spearman correlations of placental proinflammatory cytokine, tumor necrosis factor-α (Tnfa), gene expression correlated to placental clock (Clock, Bmal1, Cry1, Per1, Per2, Per3) gene expression 4 h after administration of ODN2395 or saline vehicle on gestational day 14. Data normalized to Ppia reference gene were used for correlations (2^−ΔCT, n = 6–7/group). Black circles, saline-administered pregnant rats; red squares, ODN2395-administered rats. CLOCK, circadian locomotor output cycles protein kaput; Bmal1, brain and muscle ARNT-like protein; Cry1, cryptochrome circadian regulator 1; Per1, Per2, and Per3. period circadian protein homologs 1, 2 and 3, respectively. ODN, oligodeoxynucleotides.