Table 2.
Molecular weight determination of CPP-SpyCatcher003 constructs and oligomeric forms using SEC.
| Construct | R f | V e | MW (kDa) | Column volume (ml) | Theoretical/expected MW (kDa) |
|---|---|---|---|---|---|
| SpyCatcher003 | 0.69 | 16.3 | 15.5 | 23.55 | 15.7 |
| SpyCatcher003 S49C | 0.69 | 16.3 | 15.5 | 23.55 | 15.7 |
| SpyCatcher003 S49C | 0.62 | 14.7 | 36.2 | 23.55 | — |
| CUPID-SpyCatcher003 S49C | 0.75 | 90.2 | 16.4 | 120.6 | 18.1 |
| CUPID-SpyCatcher003 S49C | 0.68 | 81.9 | 36.1 | 120.6 | — |
| CUPID-SpyCatcher003 S49C | 0.4 | 48.4 | >670 | 120.6 | — |
| Penetratin-SpyCatcher003 S49C | 0.77 | 92.8 | 12.8 | 120.6 | 18.3 |
| Penetratin-SpyCatcher003 S49C | 0.7 | 84.3 | 28.7 | 120.6 | — |
| Penetratin-SpyCatcher003 S49C | 0.4 | 48.7 | >640 | 120.6 | — |
| pVEC-SpyCatcher003 S49C | 0.76 | 91.5 | 14.5 | 120.6 | 18.3 |
| pVEC-SpyCatcher003 S49C | 0.41 | 49.9 | >670 | 120.6 | — |