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. 2023 May 13;35(8):2929–2951. doi: 10.1093/plcell/koad129

Figure 8.

Figure 8.

Phenotypes caused by CRISPR/Cas9-mediated CYP35/CYP37/CYP39/CYP45 knockout in soybean hairy roots. A) Nodulation phenotypes at 6 wpi of plants transformed with the empty vector (control) or the CYPs CRISPR vector (CYPs-KO). The first panel in each row shows the nodulated roots under white light. The second panel shows GFP fluorescence signals representing positive transgenic nodules. The third panel shows the nodules produced by a single plant. The fourth panel shows a longitudinal section of a nodule. The largest nodules at the base of the primary root were used for the sections. From left to right, scale bars = 1.5, 1.5, 1.5, and 0.3 mm. B and C) Nitrogenase activity (B) and nodule number (C) of the control and CYPs-KO hairy roots. Asterisks show significant differences determined by unpaired Student's t-tests using GraphPad Prism version 9 (**P ≤ 0.01; ***P ≤ 0.001). D and E) Relative expression of LbA and LbC1 in control and CYPs-KO hairy root nodules. Data shown in bar charts are means ± SD of 3 biological repeats.