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. 2023 May 3;35(8):2887–2909. doi: 10.1093/plcell/koad122

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© The Author(s) 2023. Published by Oxford University Press on behalf of American Society of Plant Biologists.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 6. — MdPUB24 interacts with MdNAC72. A) Y2H assay showing that MdPUB24 interacts with MdNAC72 in yeast; the assay was performed as described in Fig. 3. B) A pull-down assay showing that MdPUB24 interacts with MdNAC72 in vitro. Recombinant MdNAC72-MBP was incubated with immobilized MdPUB24-His and detected for immunoblot analysis using an anti-MBP antibody (upper panel) or an anti-His antibody (bottom panel). The asterisk represents the target protein. C) Co-IP assay showing that MdPUB24 interacts with MdNAC72 in vivo. MdPUB24-GFP and MdNAC72-FLAG were overexpressed in apple calli. An anti-FLAG antibody was used for immunoprecipitation analysis. Anti-GFP and anti-FLAG antibodies were used for immunoblotting. D) Firefly LCI assay verifying that MdPUB24 and MdNAC72 interact in N. benthamiana leaves.