FIG. 1.

(A) Schematic diagram of the predicted protein products of pCB6-EboGP and pCB6-Ebo-T. Speckled boxes represent leader peptides; hatched boxes represent TM domains. Gray shading indicates the central region of Ebo-GP that is divergent between the different strains of Ebola virus. Arrows indicate the putative endoproteolytic processing sites, as well as the Ebo-GP cytoplasmic tail. The RSV cytoplasmic tail, used as an epitope tag in Ebo-T, is indicated by an arrow; potential glycosylation sites are shown (|∨). To evaluate the incorporation of Ebo-GP and Ebo-T into MLV virions, 293T cells were transfected with MLV Gag-Pol and genome constructs with either pCB6-Ebo-GP (Ebo-GP) or pCB6-Ebo-T (Ebo-T) or without a glycoprotein construct (Mock). Viral supernatants were collected and partially purified by pelleting through 20% sucrose. Cell lysates (B) and lysed pellets (C and D) were analysed by SDS-PAGE and Western blotting with either the anti-Ebo-GP serum (B and C) or the anti-RSV tail serum (D). Arrows indicate the GP₁, GP₂, and GP₀ proteins; positions of molecular mass markers (in kilodaltons) are shown to the left of each gel.