FIG. 3.

Identification of the Ebo-GP endoproteolytic processing site. (A) Comparison of the conserved dibasic motifs in the envelope glycoproteins of all known strains of Ebola virus. (B) Comparison of the Ebo-GP endoproteolytic processing site with those of five mutant glycoproteins. (C) Western blot analysis of Ebo-T, Ebo-T.CL-1, and Ebo-T.CL(−). 293T cells were transfected with either pCB6-Ebo-T (Ebo-T), pCB6-Ebo-T.CL-1 (CL-1), or pCB6-Ebo-T.CL(−) [CL(−)] or without a glycoprotein construct (Mock). Viral lysates prepared 48 h posttransfection were analyzed by SDS-PAGE and Western blotting with the anti-RSV tail serum. Arrows indicate the GP₂ and GP₀ proteins. Positions of molecular mass markers (in kilodaltons) are shown to the left of each gel. (D) Western blot analysis of Ebo-T, Ebo-T.CL-2, Ebo-T.CL-3, and Ebo-T.CL-7. Viral preparation and analysis were performed as for panel C. Arrows indicate the GP₂ and GP₀ proteins; positions of molecular mass markers (in kilodaltons) are shown to the left of each gel.