FIG. 8.

Time course of KSHV vCYC (A) and cellular huCYC D2 (B) mRNA expression in BCBL-1 cells after release from l-mimosine G₁ arrest. GAPDH mRNA expression (C) was used as a control for equal mRNA loading. Cells were arrested for 20 h by treatment with 200 mM l-mimosine and released from cell cycle arrest by washing in fresh medium. Cell cultures were harvested at 0, 3, 6, 10, and 24 h after l-mimosine washout and prepared for mRNA extraction. Expression of KSHV LT1 and LT2 mRNA mirrors expression of cellular CYC D2, which is expressed early in G₁ phase of the cell cycle.