Extended Data Table 2.

Mutations only in non-minimal cell that that are putatively under positive selection

Simulations were performed to find genes acquiring more mutations than expected to occur by chance during 2000 generations of experimental evolution. Such mutations are indicative of positive selection. P_adj corresponds to significance following Benjamini-Hochberg correction to account for multiple comparisons. Genes are assigned to categories based on the secondary functional classifications⁴. Note that “Central metabolism” corresponds to “Central carbon metabolism” in the original source⁴. * = nonessential genes that are absent from the minimal cell, N/A = uncharacterized genes that did not fall into defined category.