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. 2023 Jul 19;620(7972):128–136. doi: 10.1038/s41586-023-06331-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a,b, Analysis of the ex vivo TCR repertoire for NQK-A8- and NQK-Q8-specific T cells after TAME on the basis of TRBV (a) and TRAV (b) use. The pie charts show the percentage of each TRBV or TRAV used in the single-cell sorted clonotypes. The CDR3β and CDR3α sequence motifs are shown below the pie charts, respectively, for the TCRs with the biased expression of TRBV7-2/7-8 paired with TRAV9/26 or TRAV21, including the public TCRs. c,d, The binding response (response units (RU)) of D5A (c) and D9A (d) TCRs (analyte) against HLA-B*15:01–NQK complexes (A8 in orange and Q8 in purple). The public TCR D5A expresses TRBV7-2 paired with TRAV21, and the public D9A TCR expresses TRBV7-2 paired with TRAV9-2; the CDR3β and CDR3α sequences for each public TCRs are shown below the binding curves. The SPR steady-state binding curves represent binding under a TCR concentration range of 0.39–100 μM. n = 2 biologically independent experiments performed in duplicate; the graph shows the results from one experiment performed in duplicate, represented by the black dots.