Table 2.

Bacterial isolates recovered from honeybees (A. mellifera), wasps (V. germanica), and hornets (V. orientalis)

s/n	Isolate code	Bacterial genus/species	Gram staining	Catalase reaction	Insect species (host)	Stinging simulation experimenta	Nutrient substrateb	Identif. methodc	NCBI accession numberd
1	3	Bacillus cereus group	+	+	A. mellifera	iv	TSA	ii	OP009593
2	4	Bacillus cereus group	+	+	A. mellifera	iv	TSA	ii	OP009594
3	5	Bacillus cereus group	+	+	A. mellifera	iv	TSA	ii	OP009595
4	6	Paenibacillus urinalis	−	+	A. mellifera	iv	TSA	v	-
5	7	Paenibacillus pabuli	−	+	A. mellifera	iv	TSA	v	-
6	8Α	Staphylococcus capitis	+	+	A. mellifera	iv	TSA	iv	-
7	8Β	Bacillus cereus group	+	+	A. mellifera	iv	TSA	ii	OP009596
8	9	Bacillus cereus group	+	+	A. mellifera	iv	TSA	ii	OP009597
9	10	Bacillus cereus group	+	+	A. mellifera	iv	TSA	ii	OP009598
10	14	Enterobacter cloacae	−	+	A. mellifera	ii	CFC	iv	-
11	15	Micrococcus luteus	+	+	A. mellifera	ii	COBA	iv	-
12	18	Staphylococcus cohnii	+	+	A. mellifera	i	BP	iv	-
13	22	Staphylococcus warneri	+	+	A. mellifera	iii	BP	iv	-
14	27	Micrococcus luteus	+	+	A. mellifera	iii	COBA	iv	-
15	28	Bacillus cereus group	+	+	A. mellifera	iii	COBA	ii	OP009599
16	31	Staphylococcus warneri	+	+	A. mellifera	i	ΒP	iv	-
17	32	Micrococcus luteus	+	+	A. mellifera	i	COBA	iv	-
18	33	Enterococcus sp.	+	−	V. germanica	iii	TSA	i	ON994583
19	34	Enterococcus mundtii	+	−	V. germanica	iii	TSA	i	ON994584
20	35	Enterococcus sp.	+	−	V. germanica	iii	TSA	i	ON994585
21	36	Enterococcus sp.	+	−	V. germanica	iii	TSA	i	ON994586
22	37	Enterococcus sp.	+	−	V. germanica	iii	TSA	i	ON994587
23	38	Staphylococcus epidermidis	+	+	V. germanica	iv	ΒP	i	ON994588
24	39	Enterococcus sp.	+	−	V. germanica	iii	KAA	i	ON994589
25	40	Enterococcus sp.	+	−	V. germanica	iii	KAA	i	ON994590
26	41	Enterococcus mundtii	+	−	V. germanica	iii	COBA	i	ON994591
27	42	Enterococcus mundtii	+	−	V. germanica	iii	COBA	i	ON994592
28	44	Proteus mirabilis	−	+	V. orientalis	iv	TSA	i	ON994593
29	46	Proteus mirabilis	−	+	V. orientalis	iv	TSA	i	ON994594
30	47	Gibbsiella dentisursi	−	+	V. orientalis	iv	TSA	i	ON994595
31	48	Enterococcus faecalis	+	−	V. orientalis	iv	KAA	i	ON994596
32	49	Staphylococcus sp.	+	+	V. orientalis	i	KAA	i	ON994597
33	50	Staphylococcus sp.	+	+	V. germanica	iv	TSA	i	ON994598
34	53	Enterobacter cancerogenus	−	+	V. germanica	iv	TSA	iii	OP009588
35	54	Enterococcus faecalis	+	−	V. germanica	iv	TSA	i	ON994599
36	55	Enterobacter cancerogenus	−	+	V. germanica	iv	CFC	iii	OP009589
37	56	Enterobacter cancerogenus	−	+	V. germanica	iv	CFC	iii	OP009590
38	57	Enterobacter cancerogenus	−	+	V. germanica	iv	CFC	iii	OP009591
39	58	Enterobacter sp.	−	+	V. germanica	i	CFC	iii	OP009592
40	59Α	Enterococcus faecalis	+	−	V. germanica	i	KAA	i	ON994600
41	59Β	Klebsiella sp.	−	+	V. germanica	i	KAA	i	ON994601
42	60	Proteus mirabilis	−	+	V. germanica	iii	CFC	i	ON994602
43	61	Proteus mirabilis	−	+	V. germanica	iii	CFC	i	ON994603
44	62	Proteus mirabilis	−	+	V. germanica	iii	CFC	i	ON994604
45	63	Proteus mirabilis	−	+	V. germanica	iii	CFC	i	ON994605
46	64	Proteus mirabilis	−	+	V. germanica	iii	CFC	i	ON994606
47	65	Proteus mirabilis	−	+	V. germanica	iii	CFC	i	ON994607
48	66	Proteus mirabilis	−	+	V. germanica	iii	CFC	i	ON994608
49	67	Proteus mirabilis	−	+	V. germanica	iii	CFC	i	ON994609
50	68	Proteus mirabilis	−	+	V. germanica	iii	COBA	i	ON994610
51	69	Enterococcus gallinarum†	−	+	V. germanica	iii	COBA	i	ON994611
52	70	Lactococcus lactis†	+	−	V. germanica	iii	COBA	i	ON994612
53	71	Enterococcus faecalis	+	−	V. germanica	iii	COBA	i	ON994613
54	72	Proteus mirabilis	−	+	V. germanica	iii	COBA	i	ON994614
55	73	Enterococcus gallinarum†	+	−	V. germanica	iii	COBA	i	ON994615
56	74	Lactococcus lactis	+	−	V. germanica	iii	COBA	i	ON994616
57	75	Enterococcus faecalis	+	−	V. germanica	iii	COBA	i	ON994617
58	76	Enterococcus faecalis	+	−	V. germanica	iii	KAA	i	ON994618
59	77	Enterococcus faecalis	+	−	V. germanica	iii	KAA	i	ON994619
60	78	Lactiplantibacillus plantarum	+	−	V. germanica	iii	KAA	i	ON994620
61	79	Proteus mirabilis	−	+	V. germanica	iii	KAA	i	ON994621
62	81	Enterococcus faecalis	+	−	V. germanica	iii	KAA	i	ON994622
63	82	Proteus mirabilis	−	+	V. germanica	iii	KAA	i	ON994623
64	83	Enterococcus faecalis	+	−	V. germanica	iii	KAA	i	ON994624

^aSee “Stinging simulation experiments”

^bsee “Nutrient substrates used to isolate the target bacteria”

^csee “Recovery of microbial isolates and identification of bacterial species”

^daccession numbers of nucleotide sequences at National Center for Biotechnology Information (NCBI) databases (https://www.ncbi.nlm.nih.gov/)

^†The primer pair 27F/1518R was used for the amplification and sequencing of the 16S rRNA genes