Fig. 6. MCT4 promotes the migration, proliferation, and aerobic glycolysis of Bca cells via miR-330-5p.

Exemplary photographs (A) and statistical diagrams (B, C) of colony formation assays were implemented using three sets of T24 or UMUC3 cells infected with circ235-overexpressing plasmids or the control using lentiviral tools, transfected with or without si-MCT4 (vector, circ235, and circ235+si-MCT4). si-MCT4 partially rescued the proliferative capacity of infected Bca cells with circ235-overexpressing plasmids using lentiviral tools. Exemplary photographs (D) and statistical diagrams (E, F) of transwell tests were used to evaluate migratory capabilities of the three sets of infected T24 and UMUC3 cells with circ235-overexpressing the plasmids or control using lentiviral tools, transfected with or without si-MCT4 (vector, circ235, and circ235+si-MCT4). si-MCT4 still partially rescued the migration of Bca cells infected with circ235-overexpressing plasmids using lentiviral tools. G, H Relative glucose consumption of three sets of Bca cells (vector, circ235, and circ235+si-MCT4) was calculated using the glucose assay kit. I, J Relative lactate production in three sets of Bca cells (vector, circ235, and circ235+si-MCT4) was detected using the lactate assay kit. K, L The ECAR of infected Bca cells with circ235-overexpressing plasmids or the control by lentiviral tools and transfected with or without si-MCT4, were quantified using a Seahorse XFe96 Extracellular Flux Analyzer. M Western blot analysis assessed PAK4, MCT4, or CPNE1 protein levels in co-transfected T24 or UMUC3 cells with si-circ235 or the control and with or without miR-330-5p inhibitor. N Western blotting testing assessed the PAK4, MCT4, or CPNE1 protein levels in T24 or UMUC3 cells that were infected with circ235-overexpressing plasmid or the control using lentiviral tools, while transfecting either miR-330-5p mimics or the control. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.