TABLE 3.
Effect of treatment with Ab to IFN-γ or IFN-α/β or both on rVV replication in nude mice
| rVV infection and treatmenta | Log10 mean virus titers ± SEMb in:
|
|
|---|---|---|
| Ovaries | Lungs | |
| VV-HA-TK | ||
| +GL113 | 8.0 ± 0.2 | 5.1 ± 0.3 |
| +anti-IFN-α/β | 8.2 ± 0.5 | 5.4 ± 0.3 |
| +anti-IFN-γ | 8.2 ± 0.1 | 5.4 ± 0.3 |
| +anti-IFN-α/β + anti-IFN-γ | 8.3 ± 0.4 | 6.0 ± 0.2c |
| VV-HA-Crg-2 | ||
| +GL113 | 6.4 ± 0.2 | 4.1 ± 0.1 |
| +anti-IFN-α/β | 7.4 ± 0.2d | 5.0 ± 0.1d |
| +anti-IFN-γ | 7.4 ± 0.2d | 5.1 ± 0.3c |
| +anti-IFN-α/β + anti-IFN-γ | 8.1 ± 0.1d | 6.1 ± 0.4d |
| VV-HA-Mig | ||
| +GL113 | 5.0 ± 0.3 | 4.0 ± 0.1 |
| +anti-IFN-α/β | 5.5 ± 0.2 | 4.7 ± 0.3c |
| +anti-IFN-γ | 5.8 ± 0.1c | 4.9 ± 0.2d |
| +anti-IFN-α/β + anti-IFN-γ | 7.5 ± 0.3d | 5.4 ± 0.2d |
a
Groups of 6- to 9-week-old female outbred nude mice were given MAb to IFN-γ or 300 U of anti IFN-α/β or both antibodies. Mice treated with MAb GL113 were used as controls. The mice were infected i.v. with 106 PFU of virus on day 0 and sacrificed 5 days later for determination of virus titers in organs. These experiments were repeated twice, with reproducible results.
b
As described for Table 2.
c
P < 0.05 compared to viral titers in control Ab-treated mice.
d
P < 0.005 compared to viral titers in control Ab-treated mice.