Fig. 4. Skp2 interacts with MLKL.

a, b Co-immunoprecipitation (Co-IP) analysis of Skp2 and MLKL interaction. 293T cells were transfected with the constructs as indicated, WCEs were subjected to Co-IP assay and Western blot analysis. c, d Co-IP analysis of endogenous Skp2 and MLKL interaction in H1299 cells. WCEs were collected and subjected to Co-IP assay and Western blot analysis. e, f Co-IP analysis of endogenous Skp2 and MLKL interaction in A549 cells. WCEs were collected and subjected to Co-IP assay and Western blot analysis. g A549 cells were treated with the indicated dose of Skp2 inhibitor SZL P1-41 for 24 h, WCEs were subjected to Co-IP assay and Western blot analysis.