FIG. 3.
Flow diagram of three cycles of single-cell subcloning of the LMH 66-1 DSL cell line. P, parental cell line used for initial subcloning. 1st, The first cycle of subcloning included 13 subclones, and subclones 5 and 8 were selected for a second round of subcloning; 2nd, six second-cycle subclones were derived from colony 5, and 12 second-cycle subclones were derived from colony 8; 3rd, third-cycle subclones were derived from second-cycle subclones P1(5)-5 and P1(5)-6, for a total of six third-cycle subclones. Open circles indicate subclones which were further subcloned, and solid circles denote subclones which were not further subcloned.