FIG. 7.

Effect of HIV infection on T-cell proliferation. Lymphocytes from uninfected cultures and cultures infected with NSI virus isolates (SV and NB) or SI virus isolates (SF2, KP, and EM) were plated on day 13 in RPMI 1640 medium with 10% FCS at 10⁵ cells/well in 96-well plates. [³H]thymidine (ICN Biomedicals, Costa Mesa, Calif.) was added to the cultures, and 10 to 16 h later, the amount of radioactivity incorporated was determined in a scintillation counter by standard procedures. Results of several separate experiments are presented and are statistically significant (NSI isolates versus control, P < 0.02; SI isolates versus control, P < 0.01; Wilcoxon signed-rank test).