TABLE 1.

Restriction sites used to generate pDI-C-derived deletion mutants

Minigenomea	Deleted nucleotidesb	Enzyme(s)	Procedure
M94	8896–9198	HpaI	Partial digestion and religation
M90	5829–6539	StuI	Religated
M88	8013–8984	NsiI	Religated
M80	3281–5050	PstI	Religated
M67	2880–5894	XcmI	Religated
M64	2880–5894	XcmI, HpaI	M67 digested with HpaI and religated
	8896–9198
M62	1363–4937	BsmI	Filled in with Klenow and religated
M54	3809–8112	NdeI	Religated (two sites within the deletion)
M50	2403–7102	AflII	Partial digestion and religation
M42	2709–8271	EcoRI	Partial digestion and religation
M39	2709–8271	EcoRI, HpaI	M42 digested with HpaI and religated
	8896–9198
M33	2713–9198	EcoRI + HpaI	EcoRI site filled in with T4 DNA pol and ligated with HpaI site
M22	1659–9198	BglII + HpaI	See CMV-M22-GUS in Materials and Methods
M21	1349–8986	SphI	Religated (two sites within the deletion)

^aThe number indicates the approximate size of the minigenome in hundreds of nucleotides. 

^bNumbering according to DI-C (47).