FIG. 2.

Tetrameric Mamu-A*01/p11C, C-M complex binds to in vitro expanded T cells from a lymph node of a SIVmac-infected, Mamu-A*01⁺ rhesus monkey. A whole-blood specimen (PBL) and a single-cell suspension of lymph node lymphocytes (LN) from a Mamu-A*01⁺ SIVmac-infected rhesus monkey (GL9) were stained with PE-coupled tetrameric Mamu-A*01/p11C, C-M complex and analyzed by flow cytometry with gating on CD8αβ⁺ CD3⁺ T cells. Cells were also expanded for 12 days in IL-2-containing medium, after stimulation either with ConA or with p11C, C-M peptide. The cells were again similarly stained and analyzed by flow cytometry.