Figure 9.

Average number of (A) total, (B) 3₁₀, (C) $\alpha$, and (D) $\pi$ helix hydrogen bonds for Acto-2 (blue) and Acto-WT (orange) as a function of the pulling distance, $\Delta r_{ee}$.The error bars for the WT (red) and mutant (blue) represent the Jarzynski weighted cumulative error over the ensemble of 100 nonequilibrium trajectories determined at the end of each stage. To see this figure in color, go online.