Fig. 2. Potency analysis of AZT against ST313. (a) Potency of 3′-azido-3′-deoxythymidine (AZT) against ST313 iNTS grown in LPM. Growth is normalized to a DMSO control (set to 100%), dots and error indicate mean and s.e.m. for three biological replicates. (b) As in panel a, for 5-fluorouracil (5-FU). (c) As in panel a, for 5-fluorocytosine (5-FC). (d) Growth kinetics of ST313 iNTS in the presence of AZT. OD600 was measured at 30 min intervals for 20 h of incubation in LPM, in the presence of AZT at the concentrations indicated. Dots and error indicate mean and s.e.m. for three biological replicates. (e) Viable bacterial counts (log CFU) of ST313 iNTS measured after 0, 2, 4, 6, and 20 h of incubation in LPM in the presence of AZT at the concentrations indicated. Dots and error indicate mean and s.e.m. for three biological replicates. (f) Table of MIC values for AZT against ST313 grown in various media conditions as indicated.