FIG. 2.

Analysis of the viral chromosomes. (A) Predicted EcoRI restriction maps of Ad5.βgal.wt and Ad5.βgal.ΔF. The 5.9-kb fragment at the right end of the Ad5.βgal.wt genome is reduced to 4.0 kb by the deletion of fiber sequences in Ad5.βgal.ΔF. (B) Ethidium bromide-stained gel of EcoRI-digested viral DNA. wt, wild-type virus; ΔF, fiberless virus. (C) Southern blot of the gel shown in panel B probed either with labeled fiber or E4 sequences. Positions of size standards (Stds) are indicated in kilobase pairs.