TABLE 3.
Complementation by BV1 and BC1 movement proteins of the accumulation of ToLCV ssDNA in protoplastsa
| A component | B component | ssDNA | dsDNA |
|---|---|---|---|
| Wild type | None | 100 | 100 |
| BV1AV2−CP− | None | 86 (50–121) | 230 (119–195) |
| BC1AV2−CP− | None | 2 (1–3) | 224 (162–288) |
| CP66:6G:BC1 | None | 5 (1–10) | 214 (180–267) |
| Wild type | Wild type | 100 | 100 |
| Wild type | BC1− | 84 (66–100) | 82 (66–98) |
| CP− | Wild type | 4 (3–6) | 164 (128–198) |
| CP− | BC1− | 5 (3–6) | 173 (160–185) |
a
Protoplasts were transfected with 2 μg of A-component DNA with or without 10 μg of B-component DNA. Viral DNA was quantitated on Southern blots with a PhosphorImager. The values represent the average amount (range) of viral DNA in two to five independent transfections, relative to a value of 100 assigned to the wild type.