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. 2023 Jun 19;24(8):e56399. doi: 10.15252/embr.202256399

Figure 1. Robust depolarization‐dependent Ub phosphorylation by PINK1 cells lacking FBXO7.

Figure 1

  • A
    Western Blot for pUb on HeLa control and FBXO7−/− cells after treatment with AO for indicated times.
  • B
    Quantification of pUb signal relative to loading. Western Blots run in triplicates from three independent clones. Error bars depict SD. Data from three biological replicates. One‐way ANOVA with multiple comparisons. **P = 0.0011; ****P < 0.0001.
  • C
    Scheme depicting production of iNeurons for biochemical analysis.
  • D
    Western Blot for pUb on extracts from day 12 iNeuron control and FBXO7−/− cells after treatment with BafA or AO for indicated times.