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. 2023 Jun 29;24(8):e57550. doi: 10.15252/embr.202357550

Figure 3. MATR3 cooperates with AS L1 RNA in chromatin organization.

Figure 3

  • A
    (Left) The representative cross‐section image showing nuclear distribution of MATR3 and H3K27me3 before and after 12 h treating with antisense L1 ASOs in AML12 cells. (Right) Line charts showing pixel intensity of each channel on the ROIs. r, coefficient of correlation. Scale bars, 5 μm.
  • B
    Standard deviation of H3K27me3 pixel intensity before (n = 98) and after (n = 97) 12 h treating with antisense L1 ASOs in AML12 cells. The P‐values were calculated using unpaired two‐tailed Student's t‐test; ****P < 0.0001. Error bars indicate mean ± s.e.m.
  • C
    (Left) The representative cross‐section image showing nuclear distribution of H3K27me3 and AS L1 RNA before and after MATR3 knockdown (Dox treatment for 3d) in AML12 cells. (Right) The normal distribution curve for the AS L1 pixel intensity. Scale bars, 5 μm.
  • D
    Skewness of antisense L1 RNA pixel intensity in Ctrl (n = 63) and shMatr3 cells (n = 66). The P‐values were calculated using unpaired two‐tailed Student's t‐test; ns, ****P < 0.0001. Error bars indicate mean ± s.e.m.
  • E
    Representative images showing nuclear colocalization of AS L1 RNAs with wild‐type and truncated GFP‐MATR3 proteins in AML12 cells. Scale bars, 5 μm.
  • F
    Coefficient of correlation between AS L1 RNA with wild‐type and truncated GFP‐MATR3 proteins. WT (n = 98), ▵ZF1 (n = 83), ▵ZF2 (n = 89), ▵RRM1 (n = 91), ▵RRM2 (n = 94). The P‐values were calculated using unpaired two‐tailed Student's t‐test; *P < 0.05, ****P < 0.0001. Error bars indicate mean ± s.e.m.
  • G
    Representative cross‐section images showing nuclear localization of H3K27me3 upon Ctrl, MATR3 knockdown and exogenous GFP‐MATR3 (WT/▵ZF1/▵ZF2/▵RRM1/▵RRM2) overexpression in AML12 cells. Scale bars, 5 μm.
  • H
    Standard deviation of H3K27me3 pixel intensity upon Ctrl (n = 50), MATR3 knockdown (n = 50), GFP‐MATR3 (WT) rescue (n = 48), GFP‐MATR3 (▵ZF1) rescue (n = 47), GFP‐MATR3 (▵ZF2) rescue (n = 46), GFP‐MATR3 (▵RRM1) (n = 45) and GFP‐MATR3 (▵RRM2) rescue (n = 49). The P‐values were calculated using unpaired two‐tailed Student's t‐test; ns, not significant, **P < 0.01, ****P < 0.0001. Error bars indicate mean ± s.e.m.

Source data are available online for this figure.