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. 2023 Jun 29;24(8):e57550. doi: 10.15252/embr.202357550

Figure EV3. AS L1 RNAs depletion affects nuclear distribution of MATR3.

Figure EV3

  • A
    Representative RNA FISH (with probes targeting antisense L1_Mus1 RNAs) and immunofluorescence images showing nuclear distribution of AS L1 RNAs and MATR3 after 24 h treating with different ASOs in AML12 cells. Scales bars, 10 μm.
  • B
    Statistical data for intracellular distribution of MATR3 proteins after 24 h treating with Scramble (n = 200), antisense L1_Mus1 (n = 198), sense L1_Mus1 (n = 195), antisense L1_MA7 (n = 200) and sense L1_MA7 (n = 200) in AML12 cells.
  • C
    The representative intracellular distribution of MATR3 proteins before and after treating with AS L1 ASOs in AML12 cells. Scales bars, 5 μm.
  • D
    Statistical data for intracellular distribution of MATR3 proteins after 0 h (n = 1,000), 6 h (n = 1,280), 12 h (n = 1,504) and 24 h (n = 1,430) treating with AS L1 ASOs in AML12 cells.
  • E, F
    The representative cross‐section image showing nuclear distribution of MATR3 and HNRNPU (E) and SAFB (F) before and after 24 h treating with antisense L1 ASOs in AML12 cells. Scales bars, 5 μm.
  • G
    (Left) The representative cross‐section image showing nuclear distribution of MATR3 and FUS before and after 24 h treating with antisense L1 ASOs in AML12 cells. (Right) The zoom‐in MERGE image showing relative distribution of MATR3 and FUS. Line charts showing pixel intensity of each channel on the ROIs. Scales bars, 5 μm.
  • H
    Scatter plot of antisense RNA expression changes in L1_Mus1 subfamily with genes expression of host between AS L1 ASO and scramble ASO treatment samples. Mus1 copies located within gene body regions are kept. X‐axis: antisense RNA expression fold changes log2(AS L1 ASO/scramble ASO) of Mus1 copies. Y‐axis: the FPKM fold changes in L1_Mus1's host genes. Pearson correlation coefficient (PCC) between antisense RNA expression changes in Mus1 copies and expression changes in host genes.