FIG. 3.

HIV-1 Env reactive 1131A5 lineage in pre-immune blood. (A) Phylogenic analysis and alignments of 11131A5 lineage. Lineage members were defined as same heavy-chain V and J gene usage, HCDR3 length, and >80% HCDR3 similarity to the mAb sequence, and clustered based on Partis analysis. Individual nodes indicate identical nucleotide sequences (n = 1–6). Black nodes indicate undefined isotype, symbol shape indicates time point. (B) Alignments depict germline, pre-immune lineage members, and M6 mAb-derived sequences. mAb, monoclonal antibody.