表3.
本方法与文献报道的比较
| No. | Sample extraction | HPLC separation | Harmful solvent | Number of reference compound |
Ref. | ||||
|---|---|---|---|---|---|---|---|---|---|
| Method | Solvent | Time/min | Mobile phase | Time/min | |||||
| 1 | RE | 50 mL methanol | 60 | 20 mL acetonitrile-0.1% (v/v) phosphoric acid aqueous solution |
20 | 50.0 mL methanol and 1.6 mL acetonitrile |
2 | [1] | |
| 2 | UE | 24 mL 94% (v/v) methanol aqueous solution |
8.5 | 50.4 mL methanol-0.1% (v/v) formic acid aqueous solution |
63 | 42.6 mL methanol | 2 | [10] | |
| 3 | RE | 50 mL methanol | 60 | 60 mL acetonitrile-0.1% (v/v) phosphoric acid aqueous solution |
60 | 50.0 mL methanol and 7.2 mL acetonitrile |
2 | [11] | |
| 4 | UE | 10 mL synthesized DES containing 20% (v/v) water | 30 | 12.5 mL methanol-0.1% (v/v) formic acid aqueous solution |
25 | 3.8 mL methanol | 2 | [12] | |
| 5 | UE | 10 mL 25% (v/v) ethanol aqueous solution |
5 | 8.25 mL acetonitrile-0.1% (v/v) formic acid aqueous solution |
5.5 | 0.5 mL acetonitrile | 1 | this method |
|
HPLC separation time was the end time of chromatogram in literature. RE: reflux extraction; UE: ultrasonic extraction; DES: deep eutectic solvent.