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. 2023 Jul 1;34(8):ar78. doi: 10.1091/mbc.E23-04-0119

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© 2023 Bailey, Surovtsev, Williams, Yan, et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial-Share Alike 4.0 International Creative Commons License.

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FIGURE 3: — Loss of cohesin or condensin activity increases chromatin mobility. (a) MSD analysis of G2 cells harboring temperature-sensitive alleles of the cohesin-loading protein Mis4 and condensin complex subunit 2 (Cut14). The fluorescently labeled mmf1 locus was imaged at the nonpermissive temperature (36°C) to inhibit G2 cohesin and condensin function, respectively. (b) The same enhancement of chromatin dynamics is observed at a separate genomic locus, pfl5. Error bars indicate standard errors of the mean. Lines are the best fit of Eq. 1 with the indicated D values.