FIGURE 5:

Impact of loop extrusion on chromatin mobility depends on the LEF lifetime. (a) MSD results of the Rouse-type polymer simulations combined with loop extrusion simulations that considered two types of LEF complexes (in equal amounts) with different lifetimes. Blue, simulations with both types of LEFs present; green, only long-lived LEF present; dark green, only short-lived LEF present; red, no LEFs. Lines represent the average over all beads and all simulations. See also Supplemental Figure S3. (b) Experimental MSDs for WT cells at 36°C (red) and cells harboring a temperature-sensitive allele of the condensin complex subunit 2 (Cut14) imaged at the nonpermissive temperature (36°C; pink) and cells harboring the temperature-sensitive allele of Cut14 imaged after depletion of the Rad21 subunit of the cohesin complex at the permissive temperature (30°C; green) and at the nonpermissive temperature (36°C; blue). Lines are the best fit of Eq. 1 with the indicated D values.