Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jul 1;34(8):br12. doi: 10.1091/mbc.E22-12-0549

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023 Liu et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial-Share Alike 4.0 International Creative Commons License.

PMC Copyright notice

FIGURE 2: — Cab45 regulates the apical secretion of exogenous PLAP-sec and endogenous clusterin/Gp80. (A) MDCK PLAP-sec knockdown for Cab45 (Cab45i) or not (CTRLi) plated on coverslip was used to reveal intracellular Cab45 and Golgi (GM130) (left panels) or PLAP-sec and ER (KDEL) (right panels). Scale bars, 10 μm. (B) Cellular media of 1 d (1D) and 4 d (4D) MDCK PLAP-sec CTRLi or Cab45i were collected as previously to monitor PLAP-sec secretion. On the right, quantification of PLAP-sec secretion normalized to intracellular tubulin levels. Experiments were performed three times. No statistical significance was observed. (C, D) Apical (AP) or basolateral (BL) media of MDCK PLAP-sec CTRLi or Cab45i plated on filters for 4 d was considered to monitor PLAP-sec secretion (C) or clusterin/Gp80 (D). On the right, quantification of AP and BL PLAP-sec (C) or clusterin/Gp80 (D) secretion normalized to intracellular tubulin levels. Experiments were performed six times; in both cases statistical significance in AP/BL secretion of PLAP-sec or clusterin/Gp80 was evaluated *, p < 0.05.