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. 2023 Jul 1;34(8):br12. doi: 10.1091/mbc.E22-12-0549

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FIGURE 4: — Overexpression of SPCA1 mutant impairing calcium uptakes leads to unpolarized secretion of PLAP-sec and clusterin/Gp80. (A) MDCK PLAP-sec stably expressing the GFP-SPCA1 mutant seeded on coverslip was used to reveal intracellular PLAP-sec localization and ER marker (top) or Golgi marker (bottom). (B, C) AP and BL media of MDCK PLAP-sec CTRL or the overexpressing GFP-SPCA1 mutant plated on filters for 4 d were collected to monitor PLAP-sec secretion (B) or clusterin/Gp80 (C). On the right, quantification of AP and BL PLAP-sec (B) or clusterin/Gp80 (C) secretion normalized to intracellular tubulin levels. Experiments were performed three times; in both cases the statistical significance in AP/BL secretion of PLAP-sec or clusterin/Gp80 was evaluated *, p < 0.05. (D) Model of calcium-dependent Cab45 mechanisms regulating apical secretion of soluble cargoes and apical sorting of GPI-APs. Cab45 plays a crucial role in the apical sorting of GPI-APs (in green) and in the apical secretion of soluble cargoes (in pink) through clustering-dependent and -independent mechanisms, respectively.