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Variables and their levels used for the CCD experiment for incubation time, pH, peptone, glucose, ferric chloride and calcium chloride
| Levels | |||||
|---|---|---|---|---|---|
| Variables | − 2 | − 1 | 0 | + 1 | + 2 |
| X1 (PH) | 2.5 | 3 | 7 | 11 | 14 |
| X2 (incubation time) | 3 | 7 | 14 | 14 | 22 |
| X3 (peptone) | 5 | 5.3 | 12.5 | 20 | 30.3 |
| X4 (glucose) | 5 | 5.3 | 12.5 | 20 | 30.3 |
| X5 (calcium chloride) | 0.1 | 0.4 | 0.6 | 0.8 | 1 |
| X16 (ferric chloride) | 0.1 | 0.4 | 0.6 | 0.8 | 1 |
