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Treatment with melatonin (24 h) inhibited the expression of the Wnt signalling pathway. (A) Expression of Wnt signal components determined by RT‐qPCR. (B) Expression of Wnt5a and β‐catenin determined by western blot analysis. (C) Expression of c‐Myc evaluated by western blot analysis. Values are mean ± SEM of duplicate experiments. *p < 0.05, **p < 0.01 and ***p < 0.001, indicate significant differences relative to the control group.
