Fig. 1.
Deletion of Lrh‐1 in the intestinal epithelium attenuates colitis‐associated colorectal cancer development. (A) Scheme of azoxymethane (AOM)‐ and dextran sodium sulphate (DSS)‐induced intestinal tumour induction. (B) Weight loss curve of control (Lrh‐1fl/fl) mice and Lrh‐1IEC KO mice after AOM treatment and during three cycles of DSS treatment. A representative experiment (n = 5 mice per group) of three repeats is shown (mean ± SD). (C) Colonic tumour development (arrows) in control mice (Lrh‐1fl/fl) and Lrh‐1IEC KO mice at day 56 following AOM/DSS treatment. (D, E) Colonic tumour numbers (D) and tumour volume (E) of control and Lrh‐1IEC KO mice (n = 7–8 per group). (F) Numbers of colonic tumours stratified according to size. (D–F) Mean ± SD of three independent experiments are shown. Unpaired Student's t‐test, *P < 0.05, **P < 0.01, ns: not significant. Experiments are repeated three times and data are pooled.