Fig. 6.

Role of LRH‐1 and Shp in growth regulation of transplanted tumour organoids. (A) Scheme of tumour organoid development and s.c. transplantation into C57Bl/6 wild‐type recipients. (B) In vivo growth of transplanted control (Lrh‐1^fl/fl) and Lrh‐1^{IEC KO} tumour organoids after s.c. transplantation into C57Bl/6 wild‐type mice. Mean values ± SD of n = 12 mice per group are shown. Experiments are repeated twice. (C) Growth of individual tumours is summarized in B. (D) Weight of isolated tumours from the experiment shown in B. (E) Examples of tumours isolated at day 24 post transplantation. (F) Histology of Lrh‐1^fl/fl and Lrh‐1^{IEC KO} tumours at day 24 post transplantation. The inlay shows magnification. Scale bars: overview 300 μm, inlay 150 μm. (G) In vivo growth of transplanted control Shp^+/+ and Shp^−/− tumour organoids after s.c. transplantation into C57Bl/6 wild‐type mice. Mean values ± SD of n = 12 mice per group are shown. Experiments are repeated twice. (H) Growth of individual tumours summarized in G. (I) Weight of isolated tumours from the experiment shown in G. (J) Examples of tumours isolated at day 24 post transplantation. (K) Histology of control Shp^+/+ and Shp^−/− tumours at day 24 post transplantation. The inlay shows magnification. Scale bars: overview 300 μm, inlay 150 μm. Mean ± SD is shown. Unpaired Student's t‐test, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.