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. 1999 Feb;73(2):1682–1688. doi: 10.1128/jvi.73.2.1682-1688.1999

FIG. 2.

FIG. 2

Packaging of UDG into viral particles is independent of the presence of Pr55Gag and Vpr. HeLa cells were cotransfected with expression plasmids encoding Pr55Gag and Vpr. Cell-free supernatant containing virus-like particles was subjected to a linear sucrose density gradient, and individual gradient fractions were collected and analyzed by Western blotting for the presence of Pr55Gag and Vpr, as well as for the presence of packaged UDG. As a control, HeLa cell extracts were analyzed by Western blotting for the presence of Pr55Gag, Vpr, and endogenous UDG.

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