Fig 4. PPP activity is impaired in the pgl mutant.

A. JE2, pgl and the complemented pgl mutant (pglcomp) were grown in CDM [1,2-¹³C]Glucose and fluxes via glycolysis and the pentose phosphate pathway (PPP) were compared as described previously [64]. The M+2 pyruvate is unique to glycolysis and the M+1 pyruvate to PPP. Thus, the M+2/M+1 ratio is indicative of carbon flux through glycolysis relative to PPP. The M+0 pyruvate can arise from different sources including the unlabeled part of the [1,2-¹³C]Glucose and pyruvogenic amino acids that are consumed alongside glucose. B. Relative levels of M+1 pyruvate indicative of PPP activity and M+2 pyruvate indicative of glycolytic activity in JE2, pgl and pgl_comp. C. The M+2/M+1 ratio indicative of pyruvate produced directly from glucose flux through glycolysis in JE2, pgl and pgl_comp. Data are the average of three independent experiments and standard deviations are shown. Significant differences were determined using ordinary one-way ANOVA with Dunnett’s multiple comparison using GraphPad Prism V9 and adjusted p-value **** p<0.0001 is indicated.