Fig. 3.

Cisplatin-treated cells exposed to additional arsenite stress have an impaired SG response. (A) RPE and OC2 cells were treated with either sodium arsenite for 1 h, 100 µM cisplatin for 25 h and either 100 µM or 50 µM cisplatin for 24 h before the co-application of 0.5 mM sodium arsenite for the last hour. Scale bars: 10 µm (main images), 5 µm (smaller images). (B) Quantification of SGs per cell and SG size for cells treated with arsenite and 50 µM cisplatin prior to arsenite. The box represents the 25–75th percentiles, and the median is indicated. The whiskers show the minimum and maximum data values, excluding outliers calculated according to Tukey's method. n=3. ***P<0.001 (unpaired two-tailed Student’s t-test).