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. 2023 Jul 5;5(3):160–169. doi: 10.1097/BS9.0000000000000167

Figure 4.

Figure 4.

Knock-down of MXRA7 influenced the characteristics of MEG-01 cells in vitro. (A) The mRNA expression of MXRA7 in PBMCs, BM cells, and MEG-01cells was detected by RT-qPCR. (B) The mRNA and protein expression of MXRA7 in MEG-01/sh-NC and MEG-01/sh-MXRA7 cells were detected by RT-qPCR and western blot, respectively. (C) The cell viability of in MEG-01/sh-NC and MEG-01/sh-MXRA7 cells was compared with colorigenic method. (D) The cell apoptosis of MEG-01/sh-NC and MEG-01/sh-MXRA7 cells were detected with flow cytometry analysis. Early apoptosis referred to Annexin-V+ 7-AAD cells, while late apoptosis referred to Annexin-V+ 7-AAD+ cells. *P <.05, **P <.01, ***P <.001. BM = bone marrow, PBMC = peripheral blood mononuclear cell, RT-qPCR = reverse transcription-quantitative real-time PCR.