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. 2023 Jul 5;5(3):160–169. doi: 10.1097/BS9.0000000000000167

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Copyright © 2023 The Authors. Published by Wolters Kluwer Health Inc., on behalf of the Chinese Medical Association (CMA) and Institute of Hematology, Chinese Academy of Medical Sciences & Peking Union Medical College (IHCAMS).

This is an open access article distributed under the Creative Commons Attribution License 4.0 (CCBY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Knock-down of MXRA7 inhibited the ERK/MAPK signaling pathway and decreased the expression of β-tubulin. MEG-01/sh-NC and MEG-01/sh-MXRA7 cells were incubated for 72 hours in the presence of 10 ng/mL rhTPO. (A) Proteins were extracted from cell lines for western blot analysis of p-AKT, AKT, p-ERK and ERK. (B) Proteins were extracted from cell lines treated with or without DMU-212 for western blot analysis of p-ERK and ERK. (C and D) Treated with or without DMU-212, the percentage and mean fluorescence (MFI) of CD41⁺ cells, and the percentage of polyploid cells (≥8N) were analyzed by flow cytometry. The expression of β-Tubulin was detected by immunofluorescence staining (E) and western blot (F). *P <.05, **P <.01, ***P <.001.